Elucidating the key environmental parameters during the production of ectoines from biogas by mixed methanotrophic consortia

Anaerobic digestion (AD) is a robust biotechnology for the valorisation of organic waste into biogas. However, rapid decrease in renewable electricity prices requires alternative uses In this context, engineering innovative platforms bio-production chemicals from CH4 has recently emerged. The extremolyte and osmoprotectant ectoine, with market price ~1000€/Kg, industrial flagship CH4-based bio-chemicals. This work aimed at optimizing accumulation ectoines using mixed microbial consortia enriched saline environments (a salt lagoon river) activated sludge, biogas as feedstock. influence NaCl (0, 3, 6, 9 12 %) Na2WO4 35 70 ?g L?1) concentrations incubation temperature (15, 25 °C) on stoichiometry kinetics methanotrophic was investigated. Consortia sludge 15 °C accumulated highest yields ectoine hydroxyectoine 6 % (105.0 ± 27.2 24.2 5.4 mgextremolyte gbiomass?1, respectively). yield (56.6 2.5 51.0 2.0 respectively) °C. supplementation tungsten to cultivation medium did not impact any consortia. A molecular characterization enrichments revealed relative abundance ectoine-accumulating methanotrophs 7–16 %, Methylomicrobium buryatense japanense main players bioconversion methane ectoine.